Elsevier

Dental Abstracts

Volume 62, Issue 2, March–April 2017, Pages 107-108
Dental Abstracts

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Ultrasonic cleaning of dentures

https://doi.org/10.1016/j.denabs.2016.07.058Get rights and content

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Background

Candida can attach to denture surface pellicle and plays a central role in biofilm formation through its ability to firmly adhere to resin. Few denture cleansers are effective against Candida, and they often contain chlorine, which can damage the metal parts of dentures. Ultrasonic cleaning is a powerful and effective cleansing method that can detach, disperse, and emulsify a dirt layer through cavitation and acceleration. Based on its ability to readily remove plaster and paraffin that is

Methods

For the in vivo section of the study, a heat-curing resin for denture bases was used to create ten 1-mm-thick specimens following manufacturer’s directions. The specimens were subjected to surface treatment, then subjected to final polishing and torqueing, and finally disinfected with a formalin sterilizer. Candida albicans and C glabrata were suspended in phosphate-buffered saline (PBS), then five specimens for each candidal species were washed three times with the PBS suspensions. The C

Results

In vitro, a mean CFU/ml count revealed significant differences between the first two and the second two stages in both the CA and the CG groups. Ultrasonic cleaning for 15 minutes in these groups removed 85.6% of the cells between stages 1 and 2 and 97.2% of the cells between stages 3 and 4.

The scanning electron microscope evaluation detected no biofilm formation in either group. The CA group demonstrated many yeast cells and some pseudohyphae clustered in grooves, pits, and pores on specimen

Discussion

Candida cells were reliably and simply removed from denture base by using ultrasonic cleaning.

Clinical Significance

Not all patients who receive dentures and oral hygiene instructions return to the dentist having achieved satisfactory results in terms of cleaning their dentures. Denture wearers often have Candida cells present in their oral cavity, even if they don’t have symptoms of colonization. As more and more of the population age and develop systemic complications, there are concerns over

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Kawaski K, Kamikawa Y, Sugihara K: In vitro and in vivo removal of oral Candida from the denture base. Gerodontology 33:247-252, 2016

Reprints available from Y Kamikawa, Dept of Maxillofacial Diagnostic and Surgical Science, Field of Oral and Maxillofacial Rehabilitation, Kagoshima Univ Graduate School of Medical and Dental Science, Sakuragaoka 8-35-1, Kagoshima 890-8544, Japan; fax: +81-92-275-6238; e-mail: [email protected]

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